If you are looking into replicating Active Directory from one machine to another
1. DCPROMO the new machine
2. Have replication transfer the database
3. Split the 5 FSMO roles from the old DC to the new DC, if so wished.
4. Profiles wont change on client machines ... profiles are SID based.
5. And of course, making the new DC a GC through ADSS ... err follow clutches link
Hmnmm, thats the basic run down, if anyone else wishes to add more, by all means :-D